![]() ![]() Mueller-Hinton broth which meets the requirements of the NCCLS standard is considered the reference medium. It allows good growth of most nonfastidious pathogens and is generally low in antagonists. MediumĬation-supplemented Mueller-Hinton broth is the most widely used medium internationally for broth dilution methods. All these methods are based on those described by Ericsson and Sherris. The broth dilution methods described in this document are essentially the same as those used in many countries including France, Germany, Sweden, the UK, and the United States. The methods described in this document are intended mainly for the testing of pure cultures of aerobic bacteria that are easily grown by overnight incubation on agar and which grow well in Mueller-Hinton broth with minimal supplementation. It is generally accepted that broth MIC tests are reproducible to within one doubling dilution of the real end point (i.e. ± one well or tube in a dilution series).īroth dilution is a technique in which containers holding identical volumes of broth with antimicrobial solution in incrementally (usually geometrically) increasing concentrations are inoculated with a known number of bacteria.īroth microdilution denotes the performance of the broth dilution test in microdilution plates with a capacity of ≤ 500 µL per well. Careful control and standardization is required for intra- and interlaboratory reproducibility as results may be significantly influenced by the method used. The MIC is a guide for the clinician to the susceptibility of the organism to the antimicrobial agent and aids treatment decisions. The lowest concentration of an antimicrobial agent (in mg/L) that, under defined in vitro conditions, prevents the appearance of visible growth of a microorganism within a defined period of time, is known as the MIC. #Serial dilution lab report microbiology seriesIn dilution tests microorganisms are tested for their ability to produce visible growth on a series of agar plates (agar dilution) or in broth (broth dilution) containing dilutions of the antimicrobial agent. MIC methods are used in resistance surveillance, the comparative testing of new agents, to establish the susceptibility of organisms that give equivocal results in disc tests, for tests on organisms where disc tests may be unreliable and when a quantitative result is required for clinical management. The tests are also important in resistance surveillance, epidemiological studies of susceptibility and in comparisons of new and existing agents.ĭilution methods are used to determine the minimum inhibitory concentrations (MICs) of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing. In vitro susceptibility tests are performed on microorganisms suspected of causing disease, particularly if the organism is thought to belong to a species that may exhibit resistance to frequently used antimicrobial agents. ![]()
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